1HWH

1:1 COMPLEX OF HUMAN GROWTH HORMONE MUTANT G120R WITH ITS SOLUBLE BINDING PROTEIN

Experimental procedure

Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1995-01
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	67.780, 67.780, 229.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.900
R-factor	0.215
Rwork	0.215
R-free	0.31700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.940
Data reduction software	MOSFLM
Data scaling software	CCP4
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.099 *	0.296 *
Number of reflections	11596
<I/σ(I)>	11
Completeness [%]	91.8	77.1 *
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.25 *	18 *	THE PROTEIN COMPLEX WAS CRYSTALLISED FROM 1.6 M LISO4 AND 50 MM BIS TRIS AT PH 6.75

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		46 (%(v/v)sat)
2	1	reservoir	PEG500	2 (%)
3	1	drop	protein	10 (mg/ml)