1HVA

ENGINEERING THE ZINC BINDING SITE OF HUMAN CARBONIC ANHYDRASE II: STRUCTURE OF THE HIS-94-> CYS APOENZYME IN A NEW CRYSTALLINE FORM

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	42.400, 72.200, 75.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.500 - 2.300
R-factor	0.155
Rwork	0.155
RMSD bond length	0.019
RMSD bond angle	3.600
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.300 *
Rmerge	0.094 *
Total number of observations	28226 *
Number of reflections	9627 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	other *	8 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	CAII	0.3 (mM)
2	1	drop	Tris-HCl	50 (mM)
3	1	drop		10 (mM)
4	1	drop		3 (mM)
5	1	reservoir	Tris-HCl	50 (mM)
6	1	reservoir		10 (mM)
7	1	reservoir		3 (mM)
8	1	reservoir	octyl beta-glucoside	1 (mM)
9	1	reservoir	ammonium sulfate	1.75-2.5 (M)