1HUY

CRYSTAL STRUCTURE OF CITRINE, AN IMPROVED YELLOW VARIANT OF GREEN FLUORESCENT PROTEIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2000-11-17
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	52.504, 61.758, 70.684
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.300 - 2.200
R-factor	0.164
Rwork	0.164
R-free	0.20800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yfp
RMSD bond length	0.009
RMSD bond angle	1.300
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.300 *	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.055	0.144
Number of reflections	12106	1181 *
<I/σ(I)>	32.6	10.3
Completeness [%]	99.3	99.7
Redundancy	4.8	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	4 *	50 mM NH4OAc, 50 mM NaOAc, 8% PEG 3400, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	PEG3400	7 (%)
3	1	reservoir		50 (mM)
4	1	reservoir		50 (mM)