1HUR

HUMAN ADP-RIBOSYLATION FACTOR 1 COMPLEXED WITH GDP, FULL LENGTH NON-MYRISTOYLATED

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1993-05-24
Detector	RIGAKU RAXIS
Spacegroup name	C 1 2 1
Unit cell lengths	121.010, 45.920, 91.780
Unit cell angles	90.00, 133.51, 90.00

Refinement procedure

Resolution	15.000 - 2.000
R-factor	0.213
Rwork	0.213
R-free	0.30900
RMSD bond length	0.018
RMSD bond angle	1.700
Data reduction software	XDS
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.000 *
Rmerge	0.078
Total number of observations	46853 *
Number of reflections	19925
Completeness [%]	82.0
Redundancy	2.35

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	13 (%)
2	1	reservoir		150 (mM)
3	1	reservoir	cacodylate	100 (mM)