1HUL

A NOVEL DIMER CONFIGURATION REVEALED BY THE CRYSTAL STRUCTURE AT 2.4 ANGSTROMS RESOLUTION OF HUMAN INTERLEUKIN-5

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	122.100, 36.110, 56.420
Unit cell angles	90.00, 98.59, 90.00

Refinement procedure

Resolution	8.000 - 2.400
R-factor	0.213
Rwork	0.213
R-free	0.36900
RMSD bond length	0.015
RMSD bond angle	3.100
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.400 *
Rmerge	0.065 *
Number of reflections	8934

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5 *	22 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	9-11 (mg/ml)
2	1	drop	Tris-HCl	0.1 (M)
3	1	drop	sodium acetate	0.25 (M)
4	1	drop	PEG4000	26-30 (%)
5	1	reservoir	Tris-HCl	0.1 (M)
6	1	reservoir	sodium acetate	0.25 (M)
7	1	reservoir	PEG4000	26-30 (%)