1HU8

CRYSTAL STRUCTURE OF THE MOUSE P53 CORE DNA-BINDING DOMAIN AT 2.7A RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	180
Detector technology	CCD
Collection date	2000-06-07
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0801
Spacegroup name	C 2 2 21
Unit cell lengths	73.676, 119.992, 184.238
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.700
R-factor	0.239 *
Rwork	0.239
R-free	0.29900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1tsr
RMSD bond length	0.007
RMSD bond angle	25.000 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000 *	2.850
High resolution limit [Å]	2.700	2.700
Rmerge	0.051	0.189 *
Total number of observations	121009 *
Number of reflections	22733
<I/σ(I)>	9.6	3.9
Completeness [%]	99.5	98.7 *
Redundancy	5.3	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	PEG 4000, KCl and MgCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	8 (%)
2	1	reservoir		200 (mM)
3	1	reservoir		50 (mM)
4	1	reservoir	dithiothreitol	10 (mM)
5	1	reservoir	Tris-HCl	50 (mM)