1HU3
MIDDLE DOMAIN OF HUMAN EIF4GII
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2000-06-28 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 0.97928 |
| Spacegroup name | H 3 |
| Unit cell lengths | 118.800, 118.800, 58.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.370 |
| R-factor | 0.239 * |
| Rwork | 0.249 |
| R-free | 0.29500 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.450 |
| High resolution limit [Å] | 2.370 | 2.370 |
| Rmerge | 0.044 | 0.195 |
| Total number of observations | 170724 * | |
| Number of reflections | 12425 | |
| Completeness [%] | 97.5 | 85 |
| Redundancy | 4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 4 * | 0.1M acetate (4.6), 0.1M ammonium acetate, 20-22% PEG4000, and 5mM tris(carboxyethyl)phosphine, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | acetate | 0.1 (M) | |
| 2 | 1 | reservoir | ammonium acetate | 0.1 (M) | |
| 3 | 1 | reservoir | PEG4000 | 20-22 (%(w/v)) | |
| 4 | 1 | reservoir | tris(carboxyethyl)phospine | 5 (mM) |
