1HTY

GOLGI ALPHA-MANNOSIDASE II

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-D
Synchrotron site	APS
Beamline	14-BM-D
Temperature [K]	100
Detector technology	CCD
Collection date	2000-05-27
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9790,0.9786,0.9770
Spacegroup name	P 21 21 21
Unit cell lengths	68.865, 109.718, 138.599
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	500.000 * - 2.140 *
R-factor	0.193 *
Rwork	0.193
R-free	0.21050 *
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.320 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.300	1.450
High resolution limit [Å]	1.760 *	1.760 *
Rmerge	0.056	0.127 *
Number of reflections	104565 *	19882 *
<I/σ(I)>	40.4	3.2
Completeness [%]	97.0 *	94.9 *
Redundancy	9.2	1.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7	298	PEG 6000, MPD, Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	Vapor diffusion *	7	298	PEG 6000, MPD, Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG6000	8.5 (%)
2	1	reservoir	MPD	5 (%)
3	1	reservoir	Tris	100 (mM)	pH7.0