1HTW
COMPLEX OF HI0065 WITH ADP AND MAGNESIUM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-11-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0642 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.350, 71.340, 95.700 |
| Unit cell angles | 90.00, 109.45, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 1.700 |
| Rwork | 0.199 |
| R-free | 0.23500 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1fl9 |
| RMSD bond length | 0.019 * |
| RMSD bond angle | 1.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.050 * | 0.120 |
| Total number of observations | 395260 * | |
| Number of reflections | 48750 | |
| <I/σ(I)> | 15 | |
| Completeness [%] | 97.0 * | 98 |
| Redundancy | 8 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 300 | PEG4000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 24 (%(w/v)) | |
| 3 | 1 | reservoir | sodium acetate | 1 (M) | |
| 4 | 1 | reservoir | 10 (mM) | ||
| 5 | 1 | reservoir | ATP | 10 (mM) | |
| 6 | 1 | reservoir | HEPES | 0.1 (M) | pH7.0 |
