1HTM
STRUCTURE OF INFLUENZA HAEMAGGLUTININ AT THE PH OF MEMBRANE FUSION
Experimental procedure
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 168.700, 231.700, 53.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 * - 2.500 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.29100 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.170 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 6.000 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 27793 |
| Completeness [%] | 81.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5 * | Bullough, P.A., (1994) J.Mol.Biol., 236, 1262. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | TBHA2 | 8-15 (mg/ml) | |
| 2 | 1 | drop | phosphate | 10 (mM) | |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | drop | sodium azide | 0.01 (%) | |
| 5 | 1 | drop | EDTA | 1 (mM) | |
| 6 | 1 | drop | citric acid | 0.1 (M) | |
| 7 | 1 | reservoir | ammonium sulfate | 55-65 (%sat) | |
| 8 | 1 | reservoir | ADA | 0.1 (M) |
