1HTL

MUTATION OF A BURIED RESIDUE CAUSES LACK OF ACTIVITY BUT NO CONFORMATIONAL CHANGE: CRYSTAL STRUCTURE OF E. COLI HEAT-LABILE ENTEROTOXIN MUTANT VAL 97--> LYS

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	118.800, 98.000, 64.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.500
R-factor	0.205
Rwork	0.205
RMSD bond length	0.011
RMSD bond angle	1.710
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.500 *
Rmerge	0.113 *
Number of reflections	19608
Completeness [%]	75.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	10.1 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	cyclohexylaminopropane sufonate	100 (mM)
2	1	reservoir	PEG1000	10 (%(w/v))