1HTI

CRYSTAL STRUCTURE OF RECOMBINANT HUMAN TRIOSEPHOSPHATE ISOMERASE AT 2.8 ANGSTROMS RESOLUTION. TRIOSEPHOSPHATE ISOMERASE RELATED HUMAN GENETIC DISORDERS AND COMPARISON WITH THE TRYPANOSOMAL ENZYME

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	65.810, 75.390, 92.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.800
R-factor	0.167
Rwork	0.167
RMSD bond length	0.019
RMSD bond angle	3.800
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.800 *
Rmerge	0.055 *
Number of reflections	9625 *
Completeness [%]	85.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4 (mg/ml)
2	1	reservoir	HEPES	100 (mM)
3	1	reservoir	PEG4000	20 (%)
4	1	reservoir	2-propanol	10 (%)
5	1	reservoir	dithiothreitol	2 (mM)