1HT9

DOMAIN SWAPPING EF-HANDS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1997-09-09
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	32.605, 48.241, 41.530
Unit cell angles	90.00, 96.98, 90.00

Refinement procedure

Resolution	20.000 - 1.760
R-factor	0.162 *
Rwork	0.162
R-free	0.19200
Structure solution method	SIRAS, MOLECULAR REPLACEMENT
Starting model (for MR)	4icb
RMSD bond length	0.011
RMSD bond angle	22.700 *
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MLPHARE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.000	1.800
High resolution limit [Å]	1.750	1.750
Rmerge	0.060	0.311
Total number of observations	50352 *
Number of reflections	11923 *
<I/σ(I)>	14.5	3.7
Completeness [%]	91.5	60.1 *
Redundancy	4.2	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5 *	293	ammonium sulphate, calcium chloride, magnesium chloride, EVAPORATION, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	35-40 (mg/ml)
2	1	drop		1.3-2.2 (mM)
3	1	reservoir	ammonium sulfate	60-70 (%)