1HSX

LYSOZYME GROWN AT BASIC PH AND ITS LOW HUMIDITY VARIANT

Experimental procedure

Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1997-06-03
Spacegroup name	P 21 21 21
Unit cell lengths	30.487, 59.583, 68.659
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.900
R-factor	0.19
Rwork	0.190
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	NATIVE TETRAGONAL FORM
RMSD bond length	0.014
RMSD bond angle	25.100 *
Data reduction software	XDS
Data scaling software	XDS
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.099	0.406
Total number of observations	72266 *
Number of reflections	10287
<I/σ(I)>	26.7	4.8
Completeness [%]	99.3
Redundancy	7.08	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	9.3 *		pH 9.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	3 (%(w/v))
2	1	1		2 (%(w/v))	pH9.3