1HSW

LYSOZYME (MUCOPEPTIDE N-ACETYLMURAMYL HYDROLASE)

Experimental procedure

Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1997-05-11
Spacegroup name	P 21 21 21
Unit cell lengths	30.496, 55.387, 68.854
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.199
Rwork	0.199
R-free	0.29100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	NATIVE ORTHORHOMBIC FORM
RMSD bond length	0.016
RMSD bond angle	25.400 *
Data reduction software	XDS
Data scaling software	XDS
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.057	0.301
Total number of observations	35416 *
Number of reflections	7683
<I/σ(I)>	26.93	4.19
Completeness [%]	92.1	90.1
Redundancy	4.65	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	9.3 *		pH 9.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	3 (%(w/v))
2	1	1		2 (%(w/v))	pH9.3