1HSS

0.19 ALPHA-AMYLASE INHIBITOR FROM WHEAT

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1995-12
Detector	RIGAKU
Spacegroup name	P 31
Unit cell lengths	79.300, 79.300, 60.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.060
R-factor	0.187
Rwork	0.187
R-free	0.22300
Structure solution method	ISOMORPHOUS REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.400
Data reduction software	RIGAKU (SOFTWARE)
Data scaling software	RIGAKU
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.070
Rmerge	0.057	0.150 *
Total number of observations	49815 *
Number of reflections	22687
Completeness [%]	81.9	67.3 *
Redundancy	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7	4 *	Miyazaki, T., (1994) J.Biochem.(Tokyo), 115, 179. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	2	PIPES	20 (mM)
2	1	2		100 (mg/ml)
3	1	1	protein	10 (mg/ml)