1HSR

BINDING MODE OF BENZHYDROXAMIC ACID TO ARTHROMYCES RAMOSUS PEROXIDASE

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Detector technology	IMAGE PLATE
Detector	FUJI
Spacegroup name	P 42 21 2
Unit cell lengths	74.500, 74.500, 118.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 1.600
R-factor	0.187
Rwork	0.187
R-free	0.22700 *
RMSD bond length	0.018
RMSD bond angle	0.049 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.500 *
Rmerge	0.047 *
Total number of observations	225152 *
Number of reflections	47193 *
Completeness [%]	87.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	22-24 *	repeated seeding, Kunishima, N., (1993) Proteins: Struct.,Funct., Genet., 15, 216. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ARP	20-40 (mg/ml)
2	1	reservoir	ammonium sulfate	32 (%sat)
3	1	reservoir	Tris-HCl	20 (mM)