1HSJ

SARR MBP FUSION STRUCTURE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	95
Detector technology	CCD
Collection date	2000-11-12
Spacegroup name	P 1
Unit cell lengths	64.739, 70.639, 75.473
Unit cell angles	65.72, 67.18, 69.58

Refinement procedure

Resolution	19.950 - 2.300
R-factor	0.2324 *
Rwork	0.263
R-free	0.28220 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Maltose binding protein 4mbp
RMSD bond length	0.009
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.370
High resolution limit [Å]	2.280	2.280
Rmerge	0.028	0.449
Total number of observations	77545 *
Number of reflections	37403 *
<I/σ(I)>	25.1	2.3
Completeness [%]	96.5 *	84.8
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	beta-mercaptoethanol	5 (mM)
3	1	reservoir	sodium acetate	100 (mM)
4	1	reservoir		100 (mM)
5	1	reservoir	PEGmme2000	18-22 (%)