1HRT

THE STRUCTURE OF A COMPLEX OF BOVINE ALPHA-THROMBIN AND RECOMBINANT HIRUDIN AT 2.8 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	C 2 2 21
Unit cell lengths	59.110, 102.620, 143.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.800
R-factor	0.155
RMSD bond length	0.019
RMSD bond angle	0.041
Refinement software	PROFFT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	2.600 *	2.600 *
Rmerge	0.099 *
Total number of observations	19008 *
Number of reflections	7688 *
Completeness [%]		13 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		22 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	29 (%)
2	1	reservoir	sodium phosphate	250 (mM)
3	1	reservoir		94 (mM)
4	1	reservoir		0.005 (%)
5	1	drop	thrombin-hirudin	7 (mg/ml)