1HRO

MOLECULAR STRUCTURE OF A HIGH POTENTIAL CYTOCHROME C2 ISOLATED FROM RHODOPILA GLOBIFORMIS

Experimental procedure

Spacegroup name	I 41
Unit cell lengths	79.200, 79.200, 75.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 2.200
R-factor	0.187 *
Rwork	0.187
RMSD bond length	0.016
RMSD bond angle	2.380
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000 *
High resolution limit [Å]	2.200 *
Rmerge	0.037 *
Completeness [%]	59562.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	ammonium sulfate	2.4 (M)
3	1	drop	MPD	3 (%)
4	1	drop	MES	50 (mM)
5	1	drop	sodium azide	5 (mM)
6	1	reservoir	ammonium sulfate	2.4 (M)
7	1	reservoir	MPD	3 (%)
8	1	reservoir	MES	50 (mM)
9	1	reservoir	sodium azide	5 (mM)