1HRM
THE PROXIMAL LIGAND VARIANT HIS93TYR OF HORSE HEART MYOGLOBIN
Experimental procedure
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.360, 28.900, 35.960 |
| Unit cell angles | 90.00, 107.06, 90.00 |
Refinement procedure
| Resolution | 5.000 - 1.700 |
| R-factor | 0.151 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 0.034 |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 8.000 * |
| High resolution limit [Å] | 1.700 * |
| Rmerge | 0.057 * |
| Total number of observations | 29784 * |
| Number of reflections | 11147 |
| Completeness [%] | 75.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 60 (%sat) | |
| 3 | 1 | drop | Tris-HCl | 20 (mM) | |
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 65-66 (%sat) | |
| 6 | 1 | reservoir | Tris-HCl | 20 (mM) | |
| 7 | 1 | reservoir | EDTA | 1 (mM) |
