1HQQ
MINIPROTEIN MP-2 (M9A) COMPLEX WITH STREPTAVIDIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | MACSCIENCE |
Temperature [K] | 108 |
Detector technology | IMAGE PLATE |
Collection date | 2000-10-20 |
Detector | MACSCIENCE |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.133, 89.299, 58.137 |
Unit cell angles | 90.00, 90.57, 90.00 |
Refinement procedure
Resolution | 20.840 - 1.700 |
Rwork | 0.224 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Mp-2 streptavidin complex |
RMSD bond length | 0.004 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.840 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.086 | 0.302 |
Number of reflections | 163318 | |
<I/σ(I)> | 8.1 | 4.2 |
Completeness [%] | 96.6 | 84.8 |
Redundancy | 4.46 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 100 mM potassium acetate, ammonium sulfate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |