1HQO
CRYSTAL STRUCTURE OF THE NITROGEN REGULATION FRAGMENT OF THE YEAST PRION PROTEIN URE2P
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-11-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9791, 0.9789, 0.9686 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.310, 69.190, 149.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.221 * |
| Rwork | 0.221 |
| R-free | 0.27500 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 22.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.048 | 0.320 |
| Total number of observations | 168715 * | |
| Number of reflections | 29499 * | |
| <I/σ(I)> | 15.4 | |
| Completeness [%] | 97.6 | 99.5 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 * | 293 | NaCl, Bicine, Tris-HCl, alpha-chymotrypsin, pH 8.8, VAPOR DIFFUSION, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3.25 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | drop | 0.3 (M) | ||
| 4 | 1 | drop | alpha-chymotrypsin | 0.01-0.001 (mg/ml) | |
| 5 | 1 | reservoir | 2.5-3.0 (M) | ||
| 6 | 1 | reservoir | Bicine | 100 (mM) |
