1HQ4
STRUCTURE OF NATIVE CATALYTIC ANTIBODY HA5-19A4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-07-26 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.900, 88.700, 72.900 |
| Unit cell angles | 90.00, 91.10, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.26520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cf8 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 27.240 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 25376 | |
| <I/σ(I)> | 13.7 | 4 |
| Completeness [%] | 97.9 | 90.5 |
| Redundancy | 2.9 | 1.105 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.2 | 298 | PEG 6000, cadmium chloride, hepes, ph 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
