1HPU
5'-NUCLEOTIDASE (CLOSED FORM), COMPLEX WITH AMPCP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-10-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8445 |
| Spacegroup name | P 1 |
| Unit cell lengths | 89.400, 89.960, 96.260 |
| Unit cell angles | 110.79, 106.43, 107.76 |
Refinement procedure
| Resolution | 28.590 - 1.850 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ush |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.038 | 0.390 |
| Number of reflections | 203784 | |
| <I/σ(I)> | 14.7 | |
| Completeness [%] | 97.1 | 93.2 |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6 | 298 | Knofel, T., (2001) J.Mol.Biol., 309, 255. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium citrate | 100 (mM) | pH6.0 |
| 2 | 1 | reservoir | 80 (mM) | ||
| 3 | 1 | reservoir | 2 (mM) | ||
| 4 | 1 | reservoir | AMPCP | 2 (mM) | |
| 5 | 1 | reservoir | PEG5000 MME | 15 (%(w/v)) | |
| 6 | 1 | reservoir | ethylenediol | 15 (%(v/v)) |
