1HP5
STREPTOMYCES PLICATUS BETA-N-ACETYLHEXOSAMINIDASE COMPLEXED WITH INTERMEDIATE ANALOUGE NAG-THIAZOLINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-05-17 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.03 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 133.621, 133.621, 174.194 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.830 - 2.100 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.22200 |
| Structure solution method | Difference Fourier |
| Starting model (for MR) | 1hp4 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 22.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.084 | 0.263 |
| Total number of observations | 361546 * | |
| Number of reflections | 52210 | |
| <I/σ(I)> | 26.4 | 6.64 |
| Completeness [%] | 96.6 | 92 |
| Redundancy | 12 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | Ammonium sulfate, tri-sodium citrate, glycerol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 2.2 (M) | |
| 3 | 1 | reservoir | trisodium citrate | 100 (mM) | pH6.0 |
| 4 | 1 | reservoir | glycerol | 20-25 (%) |
