1HP4
CRYSTAL STRUCTURE OF STREPTOMYCES PLICATUS BETA-N-ACETYLHEXOSAMINIDASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-09-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9797, 0.9795, 0.9496 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 133.100, 133.100, 176.789 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.190 - 2.200 |
| R-factor | 0.181 |
| Rwork | 0.181 |
| R-free | 0.20900 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.031 | 0.067 |
| Total number of observations | 416806 * | |
| Number of reflections | 47413 | 3854 * |
| <I/σ(I)> | 56.1 | 21.6 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 12 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6 | 298 | Ammonium sulphate, tri-sodium citrate, 20% glycerol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 2.2 (M) | |
| 2 | 1 | reservoir | trisodium citrate | 100 (mM) | pH6.0 |
| 3 | 1 | reservoir | glycerol | 20-25 (%) | |
| 4 | 1 | drop | protein | 10 (mg/ml) |
