1HP1
5'-NUCLEOTIDASE (OPEN FORM) COMPLEX WITH ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-03-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8468 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 83.700, 83.700, 181.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 1.700 |
| R-factor | 0.176 |
| Rwork | 0.176 |
| R-free | 0.19900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ush |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.690 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.044 * | 0.129 * |
| Total number of observations | 287845 * | |
| Number of reflections | 71863 | |
| <I/σ(I)> | 21.7 | |
| Completeness [%] | 98.1 | 92.2 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.6 | 298 | Knofel, T., (2001) J.Mol.Biol., 309, 255. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 60 (%sat) | ||
| 2 | 1 | reservoir | sodium acetate | 0.1 (M) | pH6.6 |
| 3 | 1 | reservoir | 0.3 (M) |
