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STRUCTURE OF GUANINE NUCLEOTIDE (GPPCP) COMPLEX OF ADENYLOSUCCINATE SYNTHETASE FROM E. COLI AT PH 6.5 AND 25 DEGREES CELSIUS
Experimental procedure
Temperature [K] | 298 |
Detector technology | AREA DETECTOR |
Collection date | 1993-12-19 |
Detector | SIEMENS-NICOLET X100 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.600, 92.910, 119.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 5.000 - 2.300 |
R-factor | 0.185 |
Rwork | 0.185 |
R-free | 0.21000 |
RMSD bond length | 0.014 |
RMSD bond angle | 24.200 * |
Data reduction software | XENGEN |
Data scaling software | XENGEN |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 15.000 |
High resolution limit [Å] | 2.300 |
Rmerge | 0.089 |
Total number of observations | 112059 * |
Number of reflections | 36747 |
Completeness [%] | 89.0 |
Redundancy | 3.07 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.5 | SOAK OF THE NATIVE P 21 21 21 CRYSTAL WITH GPPNP., pH 6.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | enzyme | 8 (mg/ml) | |
10 | 1 | reservoir | ammonium sulfate | 200 (mM) | |
2 | 1 | drop | imidazole | 20 (mM) | |
3 | 1 | drop | succinate | 75 (mM) | |
4 | 1 | drop | 2-mercaptoethanol | 0.07 (ml/L) | |
5 | 1 | drop | PEG3350 | 15 (%(w/w)) | |
6 | 1 | drop | acetic acid | 60 (mM) | |
7 | 1 | drop | ammonium sulfate | 120 (mM) | |
8 | 1 | reservoir | PEG3350 | 25 (%(w/w)) | |
9 | 1 | reservoir | acetic acid | 100 (mM) |