1HOC

THE THREE-DIMENSIONAL STRUCTURE OF H-2DB AT 2.4 ANGSTROMS RESOLUTION: IMPLICATIONS FOR ANTIGEN-DETERMINANT SELECTION

Experimental procedure

Spacegroup name	I 2 2 2
Unit cell lengths	92.920, 94.540, 133.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.400
R-factor	0.198
Rwork	0.198
RMSD bond length	0.017
RMSD bond angle	2.620
Phasing software	X-PLOR
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	2.400 *
Rmerge	0.092 *
Number of reflections	16880 *
Completeness [%]	78.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	H-2Db complex	5-10 (mg/ml)
2	1	drop	Tris-HCl	0.1 (M)
3	1	drop		1.7 (M)
4	1	reservoir	Tris-HCl	0.1 (M)
5	1	reservoir		1.7 (M)