1HO8
CRYSTAL STRUCTURE OF THE REGULATORY SUBUNIT H OF THE V-TYPE ATPASE OF SACCHAROMYCES CEREVISIAE
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-11-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 117.342, 117.342, 119.873 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 71.000 - 2.950 |
| R-factor | 0.223 |
| Rwork | 0.221 |
| R-free | 0.31200 |
| Structure solution method | SIR AND MAD |
| Starting model (for MR) | vma13 poly alanine |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.900 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA ((SCALA)) |
| Phasing software | MLPHARE |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.000 | 3.300 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.077 | 0.454 |
| Total number of observations | 87426 * | |
| Number of reflections | 20090 | |
| <I/σ(I)> | 7.4 | 1.7 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 4.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | Sagermann, M., (2000) Acta Crystallogr., Sect.D, 56, 475. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 50 (mM) | |
| 2 | 1 | drop | 100 (mM) | ||
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | protein | 10 (mg/ml) | |
| 6 | 1 | reservoir | ammonium sulfate | ||
| 7 | 1 | reservoir | Tris-HCl | 50 (mM) | |
| 8 | 1 | reservoir | dithiothreitol | 5 (mM) |
