1HNU
CRYSTAL STRUCTURE OF PEROXISOMAL DELTA3-DELTA2-ENOYL-COA ISOMERASE FROM SACCHAROMYCES CEREVISIAE
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-11-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.1765, 1.1773, 1.1697, 1.1836 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 116.139, 116.139, 123.276 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.150 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.25700 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.053 | 0.474 |
| Number of reflections | 27191 | 2651 * |
| <I/σ(I)> | 16 | 2.9 |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 5.6 | 295 | MES, ammonium sulfate, 1,4-dioxane, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | MES | 0.1 (M) | |
| 2 | 1 | 1 | 1,4-dioxane | 5 (%(w/v)) | |
| 3 | 1 | 1 | ammonium sulfate | 1.4 (M) |
