1HN0
CRYSTAL STRUCTURE OF CHONDROITIN ABC LYASE I FROM PROTEUS VULGARIS AT 1.9 ANGSTROMS RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-08-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.006 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.280, 95.140, 229.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 1.900 |
| R-factor | 0.15753 |
| Rwork | 0.156 |
| R-free | 0.21300 * |
| Structure solution method | MAD and MIR |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.440 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.08) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.068 | 0.248 |
| Total number of observations | 805344 * | |
| Number of reflections | 78220 * | |
| <I/σ(I)> | 15.5 | |
| Completeness [%] | 91.3 * | 82.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | PEG 3350, amonium acetate, magnesium acetate, Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG3350 | 15 (%(w/v)) | |
| 2 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
| 3 | 1 | reservoir | magnesium acetate | 0.2 (M) | |
| 4 | 1 | reservoir | Tris | 0.1 (M) | pH8.0 |
