1HM6

X-RAY STRUCTURE OF FULL-LENGTH ANNEXIN 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	1999-10-28
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	63.644, 96.327, 127.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 1.800
Rwork	0.224
R-free	0.25900
Structure solution method	MIR AND MOLECULAR REPLACEMENT
Starting model (for MR)	1ain
RMSD bond length	0.005
RMSD bond angle	1.102
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.830
High resolution limit [Å]	1.800	1.800
Rmerge	0.082	0.599
Total number of observations	679717 *
Number of reflections	68225
<I/σ(I)>	14.6	2.1
Completeness [%]	93.0	99.4
Redundancy	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8.5	4 *	2.2 M Ammonium sulfate, 0.1 M Tris/HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	2.2 (M)
2	1	reservoir	Tris-HCl	0.1 (M)
3	1	drop	protein	28 (mg/ml)