1HLP
STRUCTURAL FEATURES STABILIZING HALOPHILIC MALATE DEHYDROGENASE FROM AN ARCHAEBACTERIUM
Experimental procedure
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 114.600, 130.600, 123.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 3.200 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| R-free | 0.28000 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.010 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 3.240 * |
| Rmerge | 0.087 * |
| Number of reflections | 12508 |
| Completeness [%] | 88.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 19 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | MPD | 58 (%) | precipitant |
| 2 | 1 | drop | hMDH | 10 (mg/ml) | |
| 3 | 1 | drop | 0.5-0.8 (M) | ||
| 4 | 1 | drop | NADH | 1 (mM) | |
| 5 | 1 | drop | sodium phospahte | 50 (mM) | |
| 6 | 1 | reservoir | MPD | 70 (%) |
