1HLO
THE CRYSTAL STRUCTURE OF AN INTACT HUMAN MAX-DNA COMPLEX: NEW INSIGHTS INTO MECHANISMS OF TRANSCRIPTIONAL CONTROL
Experimental procedure
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 108.000, 108.000, 127.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.800 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.27300 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.900 |
| Data reduction software | XDS |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.800 |
| Rmerge | 0.098 * |
| Completeness [%] | 96.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | NA CITRATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 18 (mg/ml) | |
| 2 | 1 | reservoir | 21 (%) | ||
| 3 | 1 | reservoir | sodium citrate | 100 (mM) |
