1HLG

CRYSTAL STRUCTURE OF HUMAN GASTRIC LIPASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-09
Detector	MAR scanner 345 mm plate
Spacegroup name	I 21 3
Unit cell lengths	244.330, 244.330, 244.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 3.000
Rwork	0.210
R-free	0.22800
Structure solution method	SIRAS
RMSD bond length	0.007
RMSD bond angle	24.200 *
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	CCP4
Refinement software	X-PLOR (3843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	3.100
High resolution limit [Å]	3.000	3.000
Rmerge	0.068	0.399
Number of reflections	45522
<I/σ(I)>	10.9	2
Completeness [%]	99.6	100
Redundancy	4	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5		drop consists of equal volume of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-6 (mg/ml)
2	1	reservoir	ammonium sulfate	2 (M)
3	1	reservoir	tert-butanol	1.4 (%)