1HLC

X-RAY CRYSTAL STRUCTURE OF THE HUMAN DIMERIC S-LAC LECTIN, L-14-II, IN COMPLEX WITH LACTOSE AT 2.9 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	43.600, 57.800, 108.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.900
R-factor	0.177
Rwork	0.177
RMSD bond length	0.010
RMSD bond angle	1.600
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.900 *
Rmerge	0.067 *
Total number of observations	47094 *
Number of reflections	6214 *
Completeness [%]	97.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.2 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-19 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)
3	1	drop	2-mercaptoethanol	4 (mM)
4	1	drop	lactose	20-100 (mM)
5	1	reservoir	Tris-HCl	30 (mM)
6	1	reservoir	PEG4000	30-35 (%)
7	1	reservoir	2-mercaptoethanol	4 (mM)