1HL8
CRYSTAL STRUCTURE OF THERMOTOGA MARITIMA ALPHA-FUCOSIDASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-03-03 |
| Detector | ADSC CCD |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 172.462, 172.462, 167.414 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.000 * - 2.400 |
| R-factor | 0.188 |
| Rwork | 0.185 |
| R-free | 0.22840 * |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 * | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.069 | 0.416 |
| Total number of observations | 212598 * | |
| Number of reflections | 36833 | |
| <I/σ(I)> | 8.9 | 1.8 |
| Completeness [%] | 99.3 * | 99.3 * |
| Redundancy | 5.7 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | 20 * | 18 % PEG600, 5 % JEFFAMINE, M-600 100 MM TRIS-HCL PH 8.0, PROTEIN CONC. 5 MG/ML |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5.0 (mg/ml) | |
| 2 | 1 | reservoir | PEG6000 | 18 (%(w/v)) | |
| 3 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.0 |
| 4 | 1 | reservoir | Jeffamine M-600 | 5 (%) |
