1HL5
The Structure of Holo Type Human Cu, Zn Superoxide Dismutase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-01-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.871, 172.380, 112.450 |
| Unit cell angles | 90.00, 93.45, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.780 * |
| R-factor | 0.187 |
| Rwork | 0.185 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sos |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.604 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.780 * | 1.780 * |
| Rmerge | 0.052 | 0.350 |
| Total number of observations | 904897 * | |
| Number of reflections | 265996 | |
| <I/σ(I)> | 20 | 2.7 |
| Completeness [%] | 98.6 | 96.3 |
| Redundancy | 3.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | 0.2 M CA ACETATE, 15% PEG 3350, 0.1 M TRIS PH 8.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | PEG3350 | 7.5 (%(w/v)) | |
| 3 | 1 | drop | calcium acetate | 100 (mM) | |
| 4 | 1 | drop | Tris | 50 (mM) | pH8.0 |
| 5 | 1 | reservoir | PEG3350 | 15 (%) | |
| 6 | 1 | reservoir | calcium acetate | 200 (mM) | |
| 7 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.0 |
