1HL4
The Structure of Apo Type Human Cu, Zn Superoxide Dismutase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-01-15 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 156.404, 34.978, 114.809 |
| Unit cell angles | 90.00, 112.26, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.820 |
| R-factor | 0.234 |
| Rwork | 0.232 |
| R-free | 0.28200 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sos |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.819 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.820 * | 1.820 * |
| Rmerge | 0.061 * | 0.350 |
| Total number of observations | 176234 * | |
| Number of reflections | 50360 * | |
| <I/σ(I)> | 20 | 2.7 |
| Completeness [%] | 96.1 * | 83.6 * |
| Redundancy | 3.5 * | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.6 * | 0.2M NH4CL, 20%PEG2000, 10% ETHYLENE GLYCOL, 0.1 M MES PH 5.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | PEG2000 | 10 (%) | |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | MES | 50 (mM) | pH5.6 |
| 5 | 1 | reservoir | PEG2000 | 20 (%) | |
| 6 | 1 | reservoir | 200 (mM) | ||
| 7 | 1 | reservoir | MES | 100 (mM) | pH5.6 |
