1HK3
Human serum albumin mutant r218p complexed with thyroxine (3,3',5,5'-tetraiodo-l-thyronine)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2002-07-15 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.328, 89.284, 60.526 |
Unit cell angles | 90.00, 100.80, 90.00 |
Refinement procedure
Resolution | 35.400 * - 2.800 |
R-factor | 0.203 |
Rwork | 0.203 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1e7a |
RMSD bond length | 0.008 |
RMSD bond angle | 1.270 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.400 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.070 | 0.370 |
Number of reflections | 14600 | |
<I/σ(I)> | 5.7 | 2 |
Completeness [%] | 85.1 * | 97.7 |
Redundancy | 3.1 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7 | pH 7.00 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 100 (mg/ml) | |
2 | 1 | drop | potassium phosphate | 20 (mM) | pH7.0-7.5 |