1HJV
Crystal structure of hcgp-39 in complex with chitin tetramer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2002-10-15 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 108.645, 123.321, 135.486 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.790 - 2.750 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hjx |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.950 |
| High resolution limit [Å] | 2.750 | 1.850 |
| Rmerge | 0.076 | 0.577 |
| Number of reflections | 47968 | |
| <I/σ(I)> | 11.9 | 2.4 |
| Completeness [%] | 97.6 | 85.1 |
| Redundancy | 3.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 0.2 M AMMONIUM SULFATE, 0.1 M SODIUM CITRATE PH 4.6, 25% PEG 4000, 100 MM DTT |
