1HJU

Structure of two fungal beta-1,4-galactanases: searching for the basis for temperature and pH optimum.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Collection date	2001-09-15
Spacegroup name	C 1 2 1
Unit cell lengths	107.990, 136.589, 110.879
Unit cell angles	90.00, 118.92, 90.00

Refinement procedure

Resolution	18.000 * - 2.140 *
R-factor	0.187
Rwork	0.187
R-free	0.20900 *
Structure solution method	OTHER
RMSD bond length	0.006
RMSD bond angle	22.800 *
Data scaling software	SCALEPACK
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.000	2.220
High resolution limit [Å]	2.140	2.140
Rmerge	0.076	0.211 *
Number of reflections	76436
Completeness [%]	99.9	99.6
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *		32% PEG4000 0.2M AMMONIUM SULPHATE, 0.1M TRIS PH=8.5, pH 8.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	11 (mg/ml)
2	1	reservoir	PEG4000	32 (%)
3	1	reservoir	ammonium sulfate	0.2 (M)
4	1	reservoir	Tris	0.1 (M)	pH7.5