1HJO
ATPase domain of human heat shock 70kDa protein 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-01-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.215, 72.564, 98.927 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 9.000 - 2.300 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 22.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.340 |
| High resolution limit [Å] | 2.200 | 2.300 |
| Rmerge | 0.050 | 0.205 |
| Total number of observations | 56187 * | |
| Number of reflections | 20763 | |
| <I/σ(I)> | 22.1 | 5.5 |
| Completeness [%] | 90.7 | 80.7 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 8% PEG-8000 25 MM IMIDAZOLE, PH 7.0 20MM CACL2 1MM GAMMA-S-ATP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | imidazole | 25 (mM) | |
| 2 | 1 | reservoir | PEG8000 | 8 (%) | |
| 3 | 1 | reservoir | 10 (mM) | ||
| 4 | 1 | reservoir | 10 (mM) | ||
| 5 | 1 | reservoir | gamma-S-ATP | 1 (mM) |
