1HII

COMPARATIVE ANALYSIS OF THE X-RAY STRUCTURES OF HIV-1 AND HIV-2 PROTEASES IN COMPLEX WITH CGP 53820, A NOVEL PSEUDOSYMMETRIC INHIBITOR

Experimental procedure

Detector technology	DIFFRACTOMETER
Collection date	1993-02
Detector	ENRAF-NONIUS FAST
Spacegroup name	P 21 21 21
Unit cell lengths	33.400, 64.200, 99.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.300
R-factor	0.138
RMSD bond length	0.012
RMSD bond angle	1.530 *
Data reduction software	MADNES
Phasing software	X-PLOR
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.300 *	2.300 *
Rmerge	0.051	0.190 *
Total number of observations	39279 *
Number of reflections	9517
Completeness [%]	94.3
Redundancy	4.13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	other *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	2 (mg/ml)
2	1	drop	dimethylsulfoxide	10 (mg/ml)
3	1	drop	ammonium sulfate	15-30 (%sat)
4	1	reservoir	sodium acetate	0.1 (M)
5	1	reservoir	ammonium sulfate	15-30 (%sat)