1HI5
Eosinophil-derived Neurotoxin (EDN) - Adenosine-5'-Diphosphate Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-03-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.646, 56.318, 41.771 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 27.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.064 * | |
| Total number of observations | 53920 * | |
| Number of reflections | 11749 | |
| <I/σ(I)> | 10.7 | 4.2 |
| Completeness [%] | 97.7 * | 89.9 * |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 16 * | 1.5M AMMONIUM SULFATE, 0.1M SODIUM CACODYLATE PH 6.5, 5% ETHANOL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 9 (mg/ml) | |
| 2 | 1 | drop | sodium cacodylate | 0.1 (M) | |
| 3 | 1 | drop | ammonium sulfate | 0.75 (M) | |
| 4 | 1 | drop | ethanol | 2.5 (%) | |
| 5 | 1 | reservoir | sodium cacodylate | 0.1 (M) | |
| 6 | 1 | reservoir | ammonium sulfate | 1.5 (M) | |
| 7 | 1 | reservoir | ethanol | 5 (%) |
