1HI1
RNA dependent RNA polymerase from dsRNA bacteriophage phi6 plus bound NTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 105.650, 92.700, 140.790 |
| Unit cell angles | 90.00, 101.09, 90.00 |
Refinement procedure
| Resolution | ? - 3.000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | TBA |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.000 |
| Rmerge | 0.110 |
| Number of reflections | 52305 |
| <I/σ(I)> | 9 |
| Completeness [%] | 97.7 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.7 | 293 * | Butcher, S.J., (2000) Acta Crystallogr.,Sect.D, 56, 1473. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 10 (%) | |
| 3 | 1 | reservoir | MEs | 0.1 (M) |
