1HH1

THE STRUCTURE OF HJC, A HOLLIDAY JUNCTION RESOLVING ENZYME FROM SULFOLOBUS SOLFATARICUS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2000-09-15
Detector	MARRESEARCH
Spacegroup name	P 61 2 2
Unit cell lengths	52.699, 52.699, 207.607
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.150
R-factor	0.22 *
Rwork	0.220
R-free	0.28100
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	0.036
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	SOLVE/RESOLVE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.200
High resolution limit [Å]	2.150	2.150
Rmerge	0.041 *	0.105 *
Total number of observations	121253 *
Number of reflections	10061
<I/σ(I)>	36	7.5
Completeness [%]	99.2	97
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	4.6 *		used microseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	20 (%(w/v))
2	1	reservoir	sodium acetate	0.08 (M)
3	1	reservoir	ammonium sulfate	160 (mM)
4	1	reservoir	glycerol	20 (%)