1HH1
THE STRUCTURE OF HJC, A HOLLIDAY JUNCTION RESOLVING ENZYME FROM SULFOLOBUS SOLFATARICUS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-09-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 52.699, 52.699, 207.607 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.150 |
| R-factor | 0.22 * |
| Rwork | 0.220 |
| R-free | 0.28100 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.036 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | SOLVE/RESOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.200 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.041 * | 0.105 * |
| Total number of observations | 121253 * | |
| Number of reflections | 10061 | |
| <I/σ(I)> | 36 | 7.5 |
| Completeness [%] | 99.2 | 97 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 4.6 * | used microseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 20 (%(w/v)) | |
| 2 | 1 | reservoir | sodium acetate | 0.08 (M) | |
| 3 | 1 | reservoir | ammonium sulfate | 160 (mM) | |
| 4 | 1 | reservoir | glycerol | 20 (%) |
