1HGY
CEL6A D221A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Detector | SDMS |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.600, 67.500, 53.800 |
| Unit cell angles | 76.30, 75.20, 78.40 |
Refinement procedure
| Resolution | 8.000 * - 2.200 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | WILDTYPE CEL6A |
| RMSD bond length | 0.006 |
| RMSD bond angle | 22.800 * |
| Data reduction software | MADNESS |
| Data scaling software | MADNESS |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | 2.290 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Rmerge | 0.080 | 0.158 |
| Number of reflections | 31767 | |
| <I/σ(I)> | 8.8 | 1.9 |
| Completeness [%] | 80.3 | 48.1 |
| Redundancy | 2.3 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 | Bergfors, T., (1989) J.Mol.Biol, 209, 167. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | mPEG5000 | 20 (%) | |
| 2 | 1 | reservoir | MES | 20 (mM) | pH6.0 |
| 3 | 1 | reservoir | 10 (mM) |
